Effect of Media Strength and pH on the Growth of Hairy Roots and Production of Gymnemic Acid from Gymnema Sylvestre
Gymnema sylvestre (Madhunashini) is one of the most important medicinal plants used as a crude drug for its preventive and therapeutic properties. Among other constituents of Gymnema, gymnemic acid is a major component responsible for biological and pharmacological actions. The present study deals with the influence of different media strength and initial medium pH on the growth of hairy roots and gymnemic acid production from Gymnema sylvestre. Higher strength of the media (1.5X) favoured the biomass production (114.64 g/L FW and 12.63 g/L DW) and gymnemic acid content (11.7 mg/g DW) in the tested range of 0.25, 0.50, 0.75, 1.0, 1.5 and 2.0 X strength. Among the different hydrogen ion concentration (pH) of 4.0, 4.5, 5.0, 5.5, 5.8, 6.0 and 6.5, initial medium pH of 6.0 favoured the biomass production (102.41 g/L FW and 11.52 g/L DW) and medium pH of 5.8 favoured the gymnemic acid production (11.30 mg/g DW).
K. M. Oksman-Caldentey, D. Inze, “Plant cell factories in the postgenomicera: new ways to produce designer secondary metabolites’, Trends Plant Sci. Vol. 9, pp. 433–440, 2004.
B. B. Buchanan, W. Gruissem, R. L. Jones.“Biochemistry and Molecular Biology of Plants”. American Society of Plants Physiologists, Maryland, 2000.
S. K. Agarwal, S. S. Singh, S. Verma, V. Lakshmi, A. Sharma, S. Kumar. “Chemistry and medicinal uses of Gymnema sylvestre[GurMar] leaves–a review”, IndianDrugs.Vol. 37, pp. 354–360, 2000.
S. M. Jachal “Herbal drugs as antidiabetics: an overview”, CRIPS, Vol. 3, pp. 9–11, 2002.
P. Kanetkar, R. Singhal, M. Kamat.“Gymnema sylvestre: a memoir, recentadvances in Indian herbal drug research”, J. Clin. Biochem. Nutr. Vol. 41, pp. 77–81, 2007.
H. M. Liu, F. Kiuchi, Y. Tsuda.“Isolation and structure elucidation of gymnemic acids, anti-sweet principles of Gymnema sylvestre”, Chem. Pharm. Bull. Vol. 40, pp. 1366–1375, 1992.
R. Verpoorte, A. Contin, J. Memelink, “Biotechnology for the production of plant secondary metabolites”, Phytochem. Rev. Vol. 1, pp. 13–25, 2002.
S. Ramachandra Rao, G. A. Ravishankar.“Plant cell cultures: chemical factories of secondary metabolites”, Biotechnol. Adv. Vol. 20, pp. 10–153, 2002.
N. Praveen, M. Thiruvengadam, Y.S. Yang, S.H. Kim, H.N. Murthy, I.M. Chung.“Production of gymnemic acid from hairy root cultures of Gymnema sylvestre R. Br. as influenced by polyunsaturated fatty acids
Mapana Journal of Sciences, Vol. 18, No.4 ISSN 0975-3303 38
(PUFAs) and their antioxidant activity”, Ind. Crop and Prod. Vol. 54, pp. 54-61, 2014.
P. Nagella, M. Thiruvengadam, S. J. Jung, H. N. Murthy, I. M. Chung, “Establishment of Gymnema sylvestre hairy root cultures for the production of gymnemic acid”, Acta Physiol. Plant. Vol. 35, pp. 3067–3073, 2013.
V. Veerashree, C. M. Anuradha, V. Kumar, “Elicitor-enhanced production of gymnemic acid in cell suspension cultures of Gymnema sylvestre R. Br.”, Plant Cell. Tiss. Org. Cult. Vol. 108, pp. 27–35, 2012.
A. Bakrudeen Ali Ahmed,A. S. Rao,M. V. Rao,R. M. Taha. “Production of gymnemic acid depends on medium, explants, PGRs, color lights, temperature, photoperiod, and sucrose source in batch cultures of Gymnema sylvestre”, Sci. World J, pp. 1–11, 2012.
K. Bhuvaneswari Ch, Rao, S. Gandi,A. Giri. “Abiotic elicitation of gymnemic acid in the suspension cultures of Gymnema sylvestre”, World J. Microbiol. Biotechnol.Vol. 28, pp. 741–747, 2012.
P. Nagella,I. M. Chung,H. N. Murthy “In vitro production of gymnemic acid from cell suspension cultures of Gymnema sylvestre R. Br.”, Eng. Life Sci.Vol. 11, pp. 537–540, 2011.
N. Praveen,H. N. Murthy, I. M. Chung.“Improvement of growth and gymnemic acid production by altering the macro elements concentration and nitrogen source supply in cell suspension cultures of Gymnema sylvestre R. Br.”, Ind. Crop Prod.Vol 33, pp. 282–286, 2011.
T. Murashige, F. Skoog, “A revised medium for rapid growth and bioassays with tobacco tissue cultures”, Physiol. Plantarum,Vol. 15, pp. 473–497, 1962.
G. Sivakumar,K. W. Yu,E. J. Hahn,K. Y. Paek.“Optimization of organic nutrients for ginseng hairy roots production in largescale bioreactors”, Curr. Sci.Vol. 89, pp. 641–649, 2005.
K. W. Yu, E. J. Hahn, K. Y. Paek.“Production of adventitious ginseng roots using bioreactors”, Korean J. Plant Tiss. Cult.Vol. 27, pp. 309-315, 2000.
D. K. Dougall, S. LaBrake,G. H. Whitten.“The effects of limiting nutrients, dilution rate, culture pH, and temperature on the yield constant and anthocyanin accumulation of carrot cells grown in semicontinuous chemostat cultures”, Biotechnol. Bioeng.Vol. 25, pp. 569-579, 1983.
A. Merkli,P. Christen,I. Kapetanidis.“Production of diosgenin by hairy root cultures of Trigonella foenum-graecum L.”, Plant Cell Rep.Vol. 16, pp. 632-636, 1997.
U. Mukundan,A. Rai,H. Dawda,S. Ratnaparakhi,V. Bhide.“Secondary metabolites in Agrobacterium rhizogenes mediated transformed root cultures. In: Plant Tissue Culture and Molecular Biology. Applications and Prospects. (Ed. Srivastava PS) Narosa Publishing House, New Delhi. Pp.302-331, 1998.
Praveen and Chung Effect of Media Strength and pH 39
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