Assessment of Neuropharmacological Profile of Ethanolic Extract of Lawsonia Inermis Flowers
On preliminary basis neuropharmacological profile of ethanolic extract of flowers of Lawsonia inermis was carried out. For assessing the activity of flowers on central nervous system locomotor activity using actophotometer, muscle relaxant activity using rotarod apparatus, pentobarbital-induced hypnosis and anticonvulsant activity by maximal electroshock test was performed. Ethanolic extract of flowers of Lawsonia inermis decreased the motor activity by 48.63 % and showed 49.03% noteworthy muscle relaxation along with 127.73% potentiation of pentobarbital-induced sleeping time and 66.67% decreased the duration of tonic hind leg extension of seizures activity. The results conclude that the extract of flowers of Lawsonia inermis has significant central nervous system depressant activity. Further investigations are, however, necessary to explore mechanism(s) of action involved in these pharmacological activities.
M. Singh, M. Kaur, C. B. S. Dangi and H. Singh, “Phytochemical & TLC Profile of Lawsonia Inermis (Heena),” International Journal for Pharmaceutical Research Scholars. 3(1):624-634 (2014).
S. B. Amit, N. K. Babasaheb and V. S. Rajkumar, “A phytopharmacological review on Lawsonia inermis (Linn.),” International Journal of Pharmacy & Lifesciences. 2(1): 536-541 (2011).
M. Ali and M. R, Grever, “A cytotoxic napthoquinone from Lawsonia inermis,” Fitoterapia. 69(2):181-183 (1998).
Yamsudin and H. Winarno, “The effects of Inai (Lawsonia inermis) leave extract on blood sugar level: An Experimental Study” Research Journal of. Pharmacology. 2(2):20-23 (2008).
F. Malekzadeh, “Antimicrobial activity of Lawsonia inermis”, L. Appl. Microbiol. 16:663-664 (1968).
V.K. Sharma, “Tuberculostatic activity of henna Lawsonia inermis Linn.” Tubercle. 71(4):293-296 (1990).
B.S. Nayak, G. Isitor, E. M. Davis and G. K. Pillai, “The evidence based wound healing activity of Lawsonia inermis Linn,” Phytotherapy Research. 21(9):827-831 (2007).
K. Hemalatha, H. N. Natraj and A.S. Kiran, “Hepatoprotective activity of leaves of Lawsonia alba”, Indian Journal of Natural Product 20(4): 14-17. (2004)
B. H. Ali, A. K. Bashir and M. O. M. Tanira, “Antiinflammatory, antipyretic and analgesic effects of Lawsonia inermis L. (henna) in rats,” International Journal of Experimental and Clinical Pharmacology. 51(6):356-363 (1995).
P. Arun, K.G. Purushotham, J. Jayarani and V. Kumari, “In vitro Antibacterial activity and Flavonoid contents of Lawsonia inermis (Henna),” International Journal of PharmTech Research. 2(2):1178-1181 (2010).
M. R. Natarajan and D. K. Lalitha, “Leaf extracts of Lawsonia inermis as antifungal agent,” Current Science. 56(19):1021-1022 (1987).
M. R. Iyer, S. C. Pal, V. S. Kasture and S, B. Kasture, “Effect of Lawsonia inermis on memory and behaviour mediated via Monoamine neurotransmitters,” Indian Journal of Pharmacology. 30(3):181-185 (1998).
T. Okpekon, S. Yolou, C. Gleye, et al., “Antiparasitic activities of medicinal plants used in Ivory Coast,” Journal of Ethanopharmacology. 90(1):91-97 (2004).
S. R. Munshi, T. A. Shetye and R.K. Nair, “Antifertility activity of three indigenous plant preparations,” Planta Medica. 31(1):73-75 (1977).
B. R. Mikhaeil, F. A. Badria, G. T. Maatooq and M. M. Amer, “Antioxidant and immunomodulatory constituents of henna leaves,” Zeitschrift für Naturforschung C. 59:468-476 (2004).
N. Sultana, M. I. Choudhary and A. J. Khan, “Protein glycation inhibitory activities of Lawsonia inermis and its active principles,” Journal of Enzyme Inhibition and Medicinal Chemistry. 24(1):257-261 (2009).
N. F. Syeda, S. Amreen, S. Anusha, and F. Somaiya, “Study of Antiasthmatic Activity of Ethanolic Extract of Alternanthera Sessilis,” International Journal of Pharma Research and Health Sciences. 4 (6): 1284-1290 (2016).
S. Irwin, “Comprehensive behavioral assessment: 1a A systematic quantitative procedure for assessing the behavioral and physiologic state of the mouse,” Psychopharmacologia. 13(3):222-57 (1968).
M. C. Prabhakar, “Experimental Pharmacology. Orient Longman Pvt Ltd.; Some Pharmacology Techniques,” 51-61(2007).
H. Zhang, J. Lu, Y. Zhang, Y. Zhao, J. Wei and L. Zhou, “Anticonvulsant and sedative effect of Fufang Changniu pills and probable mechanism of action in mice,” Trop J Pharm Res. 5(6):1251-1257 (2016).
N. W. Dunham and T. S. Miya, “A note on a simple apparatus for detecting neurological deficit in rats and mice,” J Am Pharmaceut Assoc. 46:208–210 (1957).
H. G. Vogel, “Drug Discovery and Evaluation-Pharmacological Assays. 2nd Edition. New York: Springer-Verlag Berlin Heidelberg. Chapter E Hypnotic activity,” 495-496 (2002)
A. Ghorbani, H. Rakhshandeh and H. R. Sadeghnia, “Potentiating Effects of Lactuca sativa on Pentobarbital-Induced Sleep,” Iran J Pharm Res. 12(2):401-406 (2013).
E. A. Swinyard, “Electrically induced convulsions. In: Experimental Models of Epilepsy. A manual for the Laboratory Worker, In Purpura, D.P., J.K., Tower, D.B., Woodbury, D.M. and Walter, R.D. Eds. New York: Raven Press,” 433 – 458 (1982).
G. N. Dakhale, S.K. Hiware, A.T. Shinde and M. S. Mahatme, “Basic biostatistics for post-graduate students,” Indian J Pharmacol. 44(4):435-42 (2012).
U. Bhosale, R. Yegnanarayan, P.Prachi, M. Zambare and R.S. Somani, “Study of CNS depressant and behavioral activity of an ethanol extract of Achyranthes Aspera (Chirchita) in mouse model,” Ann Neurosci. 18(2):44-7 (2011).
H. Fujimori, “Potentiatiom of barbital hypnosis as an evaluation method for central nervous system depressants,” Psychopharmacologia. 7:374-378 (1965).
Y. Shi, J. W. Dong, J. H. Zhao, L.N. Tang and J.J. Zhang, “Herbal Insomnia Medications that Target GABAergic Systems: A Review of the Psychopharmacological Evidence,” Current Neuropharmacology. 12(3):289-302 (2014).
S. N. Fathima, M. Rama and P. Ushasree, “Evaluation of Anticonvulsant Potential of Methanolic Extract of Stem Bark of Bombax Ceiba in Albino Mice,” Asian Journal of Pharmaceutical Research and Development. 3(6): 1-7 (2015).
Copyright (c) 2020 Mapana Journal of Sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.