Cytotoxicity Activity of Crude Extract Produced by Marine Isolate (MS19) from Sea Water collected from Bay of Bengal, India

  • P Lalitha National centre for sustainable coastal management
  • P Gayathri Green Enviro Polestar, Puducherry
  • K A Paari CHRIST (Deemed to be University)


Marine microbes comprises approximately a half of the total global biodiversity.  Sea offers an enormous resource for novel bioactive compounds and it has been classified as the largest remaining reservoir of natural molecules to be evaluated for drug activity. Marine bacteria have been received attention of researchers due to their innate potential to produce diverse compounds that attracts biological properties such as anticancer, anti-inflammatory, antimicrobial and antioxidant properties. In this study, we reported isolation of 10 strains from sea water and tested them with standard biochemical test and their potential to utilize carbon sugar, to confirm the Actinomycetes character. Among the isolates 10 and 1 was recorded to be gram positive. Carbon utilization test revealed that all the isolates utilized dextrose and none of the isolates utilized ducitol. Strain MS19 a non-motile, catalase positive, oxidase positive, and round shape strain was isolated from sea water of the Bay of Bengal. Culture characteristic studies strongly suggested that the MS19 strain belonged to the group Actinomycetes. Production of cytotoxicity compound was carried out by liquid fermentation using Zobell marine broth medium for 5 day fermentation. Crude extract tested using A549 cell line by MTT assay showed cytotoxicity activity.

Author Biographies


National centre for sustainable coastal management, Ministry of Environment, Forest and Climate change, Anna University campus, Chennai, India. Telephone: +091-9787392319


Green Enviro Polestar, Puducherry, India


Department of Life Sciences, CHRIST (Deemed to be University), Bangalore, Karnataka, India.


[1] M. L. Amador, J. Jimeno, L. Paz-Ares, H. Cortes-Funes, and M. Hidalgo. “Progress in the development and acquisition of anticancer agents from marine sources”. Ann.Oncol. Vol. 14., pp. 1607-1615, 2003.
[2] R. Jaiganesh, N. S. Sampath Kumar 2012. “Marine Bacterial Sources of Bioactive Compounds’. Adv. Food Nut. Res. pp 65, 389, 2012.
[3] M. Ananya, A. I. A. Shah. “Therapeutic potency of anticancer peptides derived from marine organism”. Inter J Engin. Appl. Scien. Vol. 2. 2013.
[4] S.W. Lowe and A. W. Lin. “Apoptosis in cancer”. Carcinog. Vol. 21, pp 485-95. 2000.
[5] M.O. Hengartner. “The biochemistry of apoptosis”. Nature 407, pp. 770-776, 2000.
[6] S. H. Kaufmann, M. O. Hengartner. “Programmed cell death: alive and well in the new millennium”. Tre. in Cel. Biolo. Vol. 11, pp.526-534, 2001.
[7] O.S. Frankfurt, A. Krishan. “Apoptosis-based drug screening and detection of selective toxicity to cancer cells”. Antica. Drug. Vol.14, pp. 555-561, 2003.
[8] G. Hacker. “The morphology of apoptosis”. Cell Tissue Res. Vol.1, pp. 5-17, 2000.
[9] A. H. Wyllie, J. F. Kerr and A. R. Currie. “Cell death: the significance of apoptosis”. Int.
Rev. Cytol.Vol. 68, pp. 251-306, 1980.
[10] R. Thirunavukkarasu, P. Pandiyan, D. Balaraman, K. Subaramaniyan, G. E. G. Jothi, Manikkam, S. B. Sadaiyappan. “Isolation of bioactive compound from marine seaweeds against
fish pathogenic bacteria Vibrio alginolyticus (VA09) and characterisation by FTIR. J.Coas. Life Medi. Vol. 1, 26–33, 2013.
[11] P. Devi, S. Wahidulla, T. Kamat, and D. L. Souza. “Screening marine organisms for antimicrobial activity against clinical pathogens”. Indi. J. Geo. Mari. Scien. Vol. 40, pp. 338–346, 2010.
[12] N. K. Asha Devi, R. Rajendran, Karthik Sundaram. “Isolation and characterization of bioactive compounds from marine bacteria”. Indi. Jour. Natu. Produc. Resour. Vol 2, 59-64, 2011.
[13] L. Mhadhebi, K. Chaieb, A. Bouraoui. “Evaluation of antimicrobial activity of organic fractions of six marine algae from tunisian mediterranean coasts”. Int. J. Pharm.Sci. Vol.4, pp. 534–537, 2012.
[14] P. Burkholder, R. Pfister, F. Leitz. “Production of a pyrrole antibiotic by amarine bacterium”. Appl. Microbiol. Vol. 14, pp. 649–653, 1966.
[15] J. W. Blunt, B. R. Copp, W. P. Hu, M. H. Munro, P. T. Northcote and M. R. Prinsep. “Marine natural products”. Nat. Prod. Rep. Vol. 25, pp. 35-94, 2008.
[16] S. L. Kin. “Discovery of novel metabolites from marine actinomycetes”. Curr. Opini. Microbiol. Vol. 9, pp. 245–251. 2006.
How to Cite
LALITHA, P; GAYATHRI, P; PAARI, K A. Cytotoxicity Activity of Crude Extract Produced by Marine Isolate (MS19) from Sea Water collected from Bay of Bengal, India. Mapana - Journal of Sciences, [S.l.], v. 17, n. 4, oct. 2018. ISSN 0975-3303. Available at: <>. Date accessed: 20 jan. 2019.
Research Articles