Assessment of DNA Damage in Peripheral Blood of Tobacco Users

  • P Manikantan CHRIST (Deemed to be University)
  • S Rajkumar G. Kuppaswamy Naidu Memorial Hospital, Coimbatore
  • P Thirunavukkarasu National Taiwan Ocean University
  • K Harishankar SRM (Deemed to be University)
Keywords: Chewing tobacco, Tobacco, Oral cancer, Comet assay, DNA damage


The smokeless tobacco has a physical powerful association with the risk of oral leukoplakia, oral submucous fibrosis, Oral squamous cell carcinoma and Squamous cell carcinoma of the head and neck. ST components exhibit genotoxicity and may alter the structure of DNA, proteins and lipids, resulting in the production of antigenicity. Present study was carried out to investigate the effects of chewing tobacco (CT) with smoking on lymphocyte DNA damage. After signing a consent form, volunteers provided blood samples (76 samples from including experimental and control subjects) to establish SCGE (comet).  Statistically significant results were obtained in experimental subjects when compared to controls. However association found with smoking had significant effect, and it can induce maximum amount of DNA damage. The genotoxic effect of chewing tobacco (CT) should be considered in addition to other known hazards for assessing health risks.

Author Biographies

P Manikantan, CHRIST (Deemed to be University)

Dept. of Life Sciences, CHRIST (Deemed to be University), Bengaluru, Karnataka

S Rajkumar, G. Kuppaswamy Naidu Memorial Hospital, Coimbatore

Senior Microbiologist, Department of Microbiology, G. Kuppaswamy Naidu Memorial Hospital, Coimbatore, Tamilnadu.

P Thirunavukkarasu, National Taiwan Ocean University

Department of Aquaculture, School of Biological Sciences, National Taiwan Ocean University, Keelung, Taiwan.

K Harishankar, SRM (Deemed to be University)

School of Biological Sciences, Dept. of Biotechnology, SRM (Deemed to be University),  Institute of Science and Technology, Kattankulathur Chennai, Tamilnadu.


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