Assessment of DNA Damage in Peripheral Blood of Tobacco Users

  • P Manikantan CHRIST (Deemed to be University)
  • S Rajkumar G. Kuppaswamy Naidu Memorial Hospital, Coimbatore
  • P Thirunavukkarasu National Taiwan Ocean University
  • K Harishankar SRM (Deemed to be University)


The smokeless tobacco has a physical powerful association with the risk of oral leukoplakia, oral submucous fibrosis, Oral squamous cell carcinoma and Squamous cell carcinoma of the head and neck. ST components exhibit genotoxicity and may alter the structure of DNA, proteins and lipids, resulting in the production of antigenicity. Present study was carried out to investigate the effects of chewing tobacco (CT) with smoking on lymphocyte DNA damage. After signing a consent form, volunteers provided blood samples (76 samples from including experimental and control subjects) to establish SCGE (comet).  Statistically significant results were obtained in experimental subjects when compared to controls. However association found with smoking had significant effect, and it can induce maximum amount of DNA damage. The genotoxic effect of chewing tobacco (CT) should be considered in addition to other known hazards for assessing health risks.

Author Biographies


Dept. of Life Sciences, CHRIST (Deemed to be University), Bengaluru, Karnataka


Senior Microbiologist, Department of Microbiology, G. Kuppaswamy Naidu Memorial Hospital, Coimbatore, Tamilnadu.


Department of Aquaculture, School of Biological Sciences, National Taiwan Ocean University, Keelung, Taiwan.


School of Biological Sciences, Dept. of Biotechnology, SRM (Deemed to be University),  Institute of Science and Technology, Kattankulathur Chennai, Tamilnadu.


[1] Anderson D, Yu TW, Phillips BJ, Schmerzer P 1994. The effect of various antioxidants and other modifying agents on oxygen-radical-generated DNA damage in human lymphocytes in the comet assay. Mutat Res, 307: 291-298.
[2] Benowitz NL, Ferrence R, Slade J, Room R, Pope M 2000. p.65-75. Nicotine toxicit. Nicotine and public health. Washington: American Public Health Association.
[3] Betti C, Davini T, Giannessi L, Loprieno N, Barale R 1995. Comparative studies by comet test and SCE analysis in human lymphocytes from 200 healthy subjects. Mutat Res, 343: 201–207.
[4] Califano J, van der Riet P, Westra W, Nawroz H, Clayman G, Piantadosi S, Corio R, Lee D , Greenberg B , Koch W and Sidransky D 1996. Genetic progression model for head and neck cancer: implications for field cancerization. Cancer Res, 56: 2488-2492.
[5] Chakradeo PP, Nair J, Bhide SV 1994. Endogenous formation of Nnitrosoproline and other N-nitrosamino acids in tobacco users. Cancer Lett, 86: 187-94.
[6] Erenmemisoglu A, Lystun H, Kartal M 1995. Carcinoma of buccal mucosa in smokeless tobacco users a preliminary study of the use of cytology for early detection. Cyiopathoh, 6: 403-40.
[7] Gollin SM 2001. Chromosomal alterations in squamous cell carcinomas of the head and neck: window to the biology of disease. Head Neck, 23:238-253.
[8] Hecht SS 2003. Tobacco carcinogens, their biomarkers and tobacco-induced cancer. Nature Rev Cancer, 3: 733-744.
[9] Jayakumar R, Sasikala K 2008. Evaluation of DNA damage in jewellery workers occupationally exposed to nitric oxide. Environ Toxi pharmacol, 26(2): 259-261
[10] Jin C, Jin Y, Wennerberg J, Åkervall J, Dictor M, Mertens F 2002. Karyotypic heterogeneity and clonal evolution in squamous cell carcinomas of the head and neck. Cancer Genet Cytogenet, 132:85-96.
[11] Lin SC, Chen YJ, Kao SY, Hsu MT, Lin CH, Yang SC, Liu TY, Chang KW 2002b. Chromosomal changes in betel-associated oral squamous cell carcinomas and their relationship to clinical parameters. Oral Oncol, 38: 266-273.
[12] Mork J, Lie AK, Glattre E, Hallmans G, Jellum E, Koskela P 2001. Human papillomavirus infection as a risk factor for squamous-cell carcinoma of the head and neck. N Engl J Med, 344:1125-1131.
[13] Nakayama T, Kodama M, Nagata C 1984. Generation of hydrogen peroxide and superoxide anion radical from cigarette smoke. Gann, 75: 95-98,.
[14] Niphadkar MP, Contractor QQ, Bhisey RA 1994. Mutagenic activity of gastric fluid from chewers of tobacco with lime. Carcinogenesis, 15: 927-31.
[15] Oh Y and Mao L 1997. Biomarkers in head and neck carcinoma. Curr Opin Oncol, 9: 247-256.
[16] Ozkul Y, Erenmemisoglu A, CQcerJs, Menevse A, Saatci C 1995. Sisterchromatid exchange inducing effect of smokeless tobacco using on T-lymphocyte chromosomes. Mulat Res, 334: 209-12.
[17] Pai CY, Hsieh LL, Tsai CW, Chiou FS, Yang CH, Hsu BD 2002. Allelic alterations at the STR markers in the buccal tissue cells of oral cancer patients and the oral epithelial cells of healthy betel quid-chewers: an evaluation of forensic applicability. Forensic Sci Int, 129: 158-167.
[18] Sardas S, Walker D, Akyol D, Karakaya AE 1995. Assessment of smoking-induced DNA damage in lymphocytes of smoking mothers of newborn infants using the alkaline single-cell gel electrophoresis technique. Mutat Res, 335: 213– 217.
[19] Singh NP, McCoy MT, Tice RR, Schneider EL 1988. A simple technique for quantification of low levels of DNA damage in individual cells. Exp Cell Res, 175:184-191.
[20] Tice RR 1995 pp. 315–339. The single cell gel/Comet assay: a microgel electrophoretic technique for the detection of DNA damage and repair in individual cells, in: D.H. Phillips, S. Venitt-Eds. Environmental mutagenesis, BIOS Scientific Publishers Limited, Oxford UK.
[21] US Department of Health and Human Services 1994. Preventing tobacco use among young people: a report of the Surgeon General. Atlanta: Centers for Disease Control and Prevention, Office on Smoking and Health.
[22] WHO 1984. Control of oral cancer in developing countries. A WHO meeting. Bull. World Health Org, 62: 817-830.
How to Cite
MANIKANTAN, P et al. Assessment of DNA Damage in Peripheral Blood of Tobacco Users. Mapana - Journal of Sciences, [S.l.], v. 17, n. 4, oct. 2018. ISSN 0975-3303. Available at: <>. Date accessed: 20 jan. 2019.
Research Articles