Vol. 2 No. 2 (2004): Mapana Journal of Sciences
Research Projects

Callus Induction and Plantlet Regeneration From Centalla Asiatica Linn

Indumathy A.
Pachaiyappa's College, Chennai
Mahalakshmi P.
Pachaiyappa's College, Chennai
C. R. Bojan
Pachaiyappa's College, Chennai

Published 2004-08-12

Keywords

  • Centalla Asiatica Linn,
  • Callus Induction,
  • Plantlet Regeneration,
  • derooted nodal segments

Abstract

Efficient plant regeneration could be obtained from the derooted nodal segments of Centella asiatica with stole buds as the explant, when cultured on MS medium supplemented with BAP (10 ppm)+NAA(2ppm). Both callus and regeneration occurred simultaneously on the same medium. Profuse rooting were obtain on MS medium fortified with NAA (2ppm) from leaf explants. Shoot and root proliferation were observed on the medium supplemented with BAP(5 ppm)and NAA (2 ppm) through subculture. Mass propagation of plantlets were obtained through invitro culture.