Isolation and Characterization of Alkalophilic Xylanase Producing Bacteria MS-2-1 from Marine Water Sample
DOI:
https://doi.org/10.12723/mjs.47.5Keywords:
Xylanase, Marinobacter, Genomic DNAAbstract
A novel marine bacterium was isolated from Pichavaram mangrove forest located near Chidambaram, Tamil Nadu and designated as MS-2-1. Morphological, cultural, physiological characteristics as well as 16S rRNA gene sequence based phylogenetic analysis confirmed taxonomic affiliation of MS 2 as Marinobacter aquaeolei. The optimum pH and temperature for maximum production of xylanase was observed at pH 8.0 and 30ºC.
References
C. E. Wyman. “What is (and is not) vital to advancing cellulosic ethanol”, Trends Biotechnol. Vol 25, p. 153, 2007.
J. E. Leach, M. L. Rhoads, C. M. Vera Cruz, F. F. White, T. W. Mew, and H. Leung. “Assessment of Genetic diversity and population structure of Xanthomonas Oryzae Pv. Oryzae with a repetitive DNA element,” Applied and Environmental Microbiology, Vol 58, No 7, pp. 2188-2195, 1992.
R. C. Kuhad and A. Singh. “Lignocellulose Biotechnology: current and future prospects. Critical Rev. Biotechnol. Vol 13, p. 151, 1993.
J. X. Heck , S. H. Flores, P. F. Hertz, and M. A. Z. Ayub. Process Biochem. Vol 40, No 107, 2005.
W. Weisberg, S. Barns, D. Pelletier, and D. Lane. “16S ribosomal DNA amplification for phylogenetic study. The Journal of Bacteriology”. Vol 173, pp. 697-703, 1991.
D. G. Higgins, A. J. Bleasby, and R. Fuchs. “CLUSTAL V: improved software for multiple sequence alignment”. Comput. Appl. Biosci. Vol 8, pp. 189-191, 1992.
G. L. Miller, R. Blum, W.E. Glennom, and A. L. Burton. “Measurement of methods for assay of xylanase activity”. Analytical Biochemistry, Vol 2, pp. 127–132, 1959.
Additional Files
Published
Issue
Section
License
Copyright (c) 2018 Asha B M, H Ananda Vardhan, Nida Afshan Khan, Sharon Eveline J
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
