Vol. 18 No. 4 (2019): Mapana Journal of Sciences
Research Articles

Effect of Media Strength and pH on the Growth of Hairy Roots and Production of Gymnemic Acid from Gymnema Sylvestre

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  • Praveen N,
  • I M Chung
Praveen N
Christ University
I M Chung
College of Life and Environmental Sciences, Konkuk University, Seoul, Republic of Korea
DOI: https://doi.org/10.12723/mjs.51.3

Published 2021-12-01

Keywords

  • Gymnema sylvestre,
  • Gymnemic Acid,
  • Madhunashini,
  • Media Strength,
  • Hydrogen Ion Concentrations

Copyright (c) 2020

Creative Commons License

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Abstract

Gymnema sylvestre (Madhunashini) is one of the most important medicinal plants used as a crude drug for its preventive and therapeutic properties. Among other constituents of Gymnema, gymnemic acid is a major component responsible for biological and pharmacological actions. The present study deals with the influence of different media strength and initial medium pH on the growth of hairy roots and gymnemic acid production from Gymnema sylvestre. Higher strength of the media (1.5X) favoured the biomass production (114.64 g/L FW and 12.63 g/L DW) and gymnemic acid content (11.7 mg/g DW) in the tested range of 0.25, 0.50, 0.75, 1.0, 1.5 and 2.0 X strength. Among the different hydrogen ion concentration (pH) of 4.0, 4.5, 5.0, 5.5, 5.8, 6.0 and 6.5, initial medium pH of 6.0 favoured the biomass production (102.41 g/L FW and 11.52 g/L DW) and medium pH of 5.8 favoured the gymnemic acid production (11.30 mg/g DW).

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