Vol. 19 No. 4 (2020): Mapana Journal of Sciences
Research Articles

Non-Recombinant Mutagenesis of Bacillus Tropicus CUIMW1718 for Hyper Production of Alginase

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  • S Induresmi,
  • PS Shiva Prasad,
  • William Joseph Kamal,
  • Maria Joy,
  • Venkatanagaraju Erumalla
S Induresmi
Department of Life Sciences, CHRIST (Deemed to be University)(CU), Bengaluru, India.
PS Shiva Prasad
Department of Life Sciences, CHRIST (Deemed to be University)(CU), Bengaluru, India
William Joseph Kamal
Department of Life Sciences, CHRIST (Deemed to be University)(CU), Bengaluru, India
Maria Joy
Department of Life Sciences, CHRIST (Deemed to be University)(CU), Bengaluru, India
Venkatanagaraju Erumalla
Department of Life Sciences, CHRIST (Deemed to be University)(CU), Bengaluru, India
DOI: https://doi.org/10.12723/mjs.55.2

Published 2020-12-02 — Updated on 2020-10-01

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Keywords

  • Industrial important, Alginase, Bacillus tropicus CUIMW1718, Non-recombinant mutagenesis

Copyright (c) 2020

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Abstract

Microbial extracellular enzymes occupied a prominent place in industrial sector due to their multifunctional ability. Extensive application of these, gap between production and demand is widening. In order to fulfil this gap, researchers focusing on various strain improvement methods such as recombinant and non-recombinant mutagenesis. In the present research work we made an attempt to screen high yielding industrial important extracellular alginase producing Bacillus tropicus CUIMW1718 strain by treating with inexpensive mutagens such as UV, EMS and EtBr. In this indigenous strain was subjected to Ultraviolet (UV) irradiation, Ethidium bromide (EtBr), Ethyl Methane Sulfonate (EMS) mutagenesis, followed by cross mutation of Ultraviolet (UV) irradiated strain with Ethyl Methane Sulfonate (EMS) and Ethidium bromide (EtBr). High yielding mutant strains were selected based on zone of clearance.

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