Statistical optimization of fermentation media for postbiotic metabolite production from Lactobacillus plantarum LG138 of primate origin
DOI:
https://doi.org/10.12723.mjs.64.9Keywords:
Exopolysaccharide, Lactic acid bacteria (LAB), Response Surface Methodology (RSM), Lactobacillus plantarum, optimizationAbstract
The probiotic metabolites called postbiotics synthesized by consist of range of molecules namely organic acids, antimicrobial peptides, short-chain fatty acids, exopolysaccharide (EPS), cofactors, vitamins, immune-modulating compounds, enzymes, neurotransmitters etc. LABs are widely recognized as an efficient EPS producer. Hence, the goal of the current study is to statistically maximize the media components for maximum exopolysaccharide production by Lactobacillus plantarum LG138 from primate feces. The de Man, Rogosa and Sharpe (MRS) medium is used for optimization of production process. Batch culture system is used for optimization of exopolysaccharide production in MRS medium using Lactobacillus plantarum LG138. The optimization process of EPS production improved its yield by 2.7-folds (from 12.00 mg/ml to 32.88 mg/ml).The enhanced EPS yield was achieved after optimization of different media components such as sucrose (5%), ammonium sulfate (1.2%), temperature (32.5⁰C), incubation time (22 h) and pH (6.5) using Response Surface Methodology. The actual experimental value (32.88 mg/ml) was comparable to the predicted maximum EP by Lactobacillus plantarum LG138.S production (32.87mg/ml) under optimal conditions for L. plantarum LG138.The in vitro antioxidant assays (free radical scavenging ability and reducing power) revealed antioxidant properties of EPS. These significant activities recommend the possible potential use of EPS in animal food and feed applications.
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