Temperature Modulated Genotoxicity in Coelomocytes of Ecologically Different Earthworm Species Exposed to Sub-lethal Doses of Zinc and Hexavalent Chromium
DOI:
https://doi.org/10.12723/mjs.69.6Keywords:
Temperature, Heavy metals, Genotoxicity, DNA strand breaks, Exacerbation in toxicityAbstract
Ecotoxicology is concerned with the possible long-term impacts of environmental stresses that alter organisms at the genetic level. Earthworm coelomocytes are sensitive environmental stress markers often employed in genotoxicity assessments. In this study, DNA damage was studied in coelomocytes of two different earthworm species, Eudrilus eugeniae, and Lampito mauritii, upon exposure to Zn2+ and Cr6+ spiked soils at variable temperatures viz., 18℃ (Cold), 24℃ (Control), 28℃ (Warm) to measure modulatory actions of temperature stress. The results of the study infer a clear species-specific response to combined toxicity. Hence, comet assay representing genotoxicity in coelomocytes is the warning signal of temperature and metal stress in earthworms. Therefore, temperature should be considered as an important factor in toxicity testing, as it indicates variations in local conditions that could affect the efficacy of heavy metal toxicity in earthworms of different ecological habitats.
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