DNA Barcoding of Fish Fauna using Mitochondrial CO1 Gene


  • Jisha Jacob Valiyaparambil University of Calicut
  • Anisha S Menon University of Calicut
  • Manoj Mathews University of Calicut
  • Nandini N J University of Kerala




DNA barcoding, DNA sequencing, Mitochondrial CO1 gene, Molecular taxonomy, Biodiversity assessment


This study aimed to investigate the quantitative relationships between four fish species from three genera based on molecular analyses (barcoding) of nine species from five genera utilizing the mitochondrial COI gene. Species within the same genus showed more transitional incompatibilities than transversional mismatches. The samples were divided into four main groups by a phylogenetic tree built from the sequencing data (cytochrome COI) of samples from the two populations using the neighbour-joining method. As dissimilar species were clustered under separate nodes and similar species were clustered under the same nodes, the neighbour-joining tree revealed various clusters corresponding to the taxonomic status of the species. In conclusion, the mitochondrial CO1 gene is a useful molecular marker for DNA barcoding.


Author Biographies

Manoj Mathews, University of Calicut

PG & Research Department of Chemistry

Nandini N J, University of Kerala

Department of Zoology


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