Vol. 24 No. 4 (2025): Mapana Journal of Sciences
Research Articles

Impact of NaCl on the morphology and biochemical aspects of Asian tiger mosquito, Aedes albopictus Skuse (Diptera: Culicidae)

  • Neethu Karan,
  • Ajitha V. S.
Neethu Karan
Department of Zoology, University College, Thiruvananthapuram, 695034, Kerala, India
Ajitha V. S.
Department of Zoology, University College, Thiruvananthapuram, 695034, Kerala, India

Published 2025-12-01

Keywords

  • Aedes albopictus,
  • NaCl,
  • LC50,
  • Morphology,
  • Biochemical assay

Copyright (c) 2025

Creative Commons License

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Abstract

The main objective of this investigation is to analyse NaCl-induced morphological and biochemical changes in 13Aedes albopictus. Ae. albopictus larvae were treated with distinct concentrations of NaCl to establish morphological as well as biochemical responses.1L3 arval mortality was recorded after 24-hour exposure period to determine the LC50 value by using Probit Analysis, IBM’s SPSS 29 Programme and Student’s t-test. The NaCl solution caused a notable mortality after 24h experimental period. The LC50 value of NaCl in Ae. albopictus was found to be 1.7% and hence the sub-lethal dose, 1.3% NaCl, was used for further analyses. Morphological studies of eggs and 7fourth instar larvae of Ae. albopictus displayed severe morphological alterations in the treated samples as contrasted with those of the untreated groups. In the fourth instar larvae of Ae. albopictus,the total protein, total free amino acid and major1a2ntioxidant enzymes such as GST and GPx were found to be increased, and38the activities of SOD, CAT and AChE were substantially declined as compared with those of the control group. NaCl adversely affects the growth and development of Ae. albopictus and leads to mortality due to metabolic as well as biochemical imbalances.

References

  1. . Khan H R, Hossain M M (2013). High temperature treatment on the eggs
  2. of the Mosquito, Culex quinquefasciatus Say, and its effects on the subsequent stages developed therefrom. Journal of the Asiatic Society of Bangladesh, Science 39(2): 247-257.
  3. . Bernhardt S A, Simmons M P, Olson K E, Beaty B J, Blair C D, Black W C (2012). Rapid intraspecific evolution of miRNA and siRNA genes in the mosquito Aedes aegypti. PloS one 7(9):1-16.
  4. . Brown A W (1986). Insecticide resistance in mosquitoes: a pragmatic review. Journal of the American Mosquito Control Association, 2(2): 123-140.
  5. . Lee H L, Lime W (1989). A re-evaluation of the susceptibility of field- collected Aedes (Stegomyia) aegypti (Linnaeus) larvae to temephos in Malaysia. Mosquito-Borne Diseases Bulletin 6(4): 91-95.
  6. . Iqbal M Z H, Bashar K, Hawlader A J (2020). Larvicidal Effect of Some Selected Salts Against the Dengue Vector Mosquito, Aedes aegypti (Diptera: Culicidae) in Bangladesh. Research Square 1:1-13.
  7. . Wei Xiang B W, Saron W A, Stewart J C, Hain A, Walvekar V, Missé D, Pompon J (2022). Dengue virus infection modifies mosquito blood- feeding behaviour to increase transmission to the host. Proceedings of the National Academy of Sciences 119(3): e2117589119.
  8. . Ward J V (1992). Aquatic insect ecology. 1. Ecology and habitat, 11:438.
  9. . Kengne P, Charmantier G, Blondeau‐Bidet E, Costantini C, Ayala D (2019). Tolerance of disease‐vector mosquitoes to brackish water and their osmoregulatory ability. Ecosphere 10(10): e02783.
  10. . Zhang M, Zhang H, Zheng J X, Mo H, Xia K F, Jian S G (2018). Functional identification of salt-stress-related genes using the FOX hunting system from Ipomoea pes-caprae. International Journal of Molecular Sciences 19(11):3446.
  11. . Parida A K, Das A B (2005). Salt tolerance and salinity effects on plants: a review. Ecotoxicology and environmental safety 60(3): 324-349.
  12. . Muchate N S, Rajurkar N S, Suprasanna P, Nikam T D (2019). NaCl induced salt adaptive changes and enhanced accumulation of 20-hydroxyecdysone in the in vitro shoot cultures of Spinacia oleracea (L.). Scientific reports 9(1): 12522.
  13. . Gayathri R A, Evans D A (2018). Culex quinquefasciatus Say larva adapts to temperature shock through changes in protein turn over and amino acid catabolism. Journal of thermal biology 74:149-159.
  14. . Huang Y M (1979). Medical entomology studies 11. The subgenus Stegomyia of Aedes in the oriental region with keys to the species (Diptera: Culicidae). Contributions of the American Entomological Institute 15: 1-76.
  15. . Knight K L, Stone A (1977). A Catalog of the Mosquitoes of the World (Diptera: Culicidae). Entomological Society of America 6:611.
  16. . Finney D J (1952). Probit analysis: a statistical treatment of the sigmoid response curve. 1-331.
  17. . Bradford M M (1976). A rapid and sensitive method for the quantitation of
  18. microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry 72(1-2): 248-254.
  19. . Spies J R (1957). Colorimetric procedures for amino acids. Methods in Enzymology 3:467-477.
  20. . Kakkar P, Das B, Viswanathan P N (1984). A modified spectrophotometric assay of superoxide dismutase. Indian Journal of Biochemistry and Biophysics 21(1): 130-132.
  21. . Maehly A C, Chance B (1954). The assay of catalases and peroxidases. Methods of biochemical analysis 1: 357-424.
  22. . Lawrence R A, Burk R F (1976). Glutathione peroxidase activity in selenium-deficient rat liver. Biochemical and biophysical research communications 71(4):952-958.
  23. . Habig W H, Pabst M J, Jakoby W B (1974). Glutathione S-transferases:
  24. the first enzymatic step in mercapturic acid formation. Journal of biological Chemistry 249(22): 7130-7139.
  25. . Ellman G L, Courtney K D, Andres Jr V, Featherstone R M (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical pharmacology 7(2): 88-95.
  26. . Suman D S, Shrivastava A R, Pant S C, Parashar B D (2011). Differentiation of Aedes aegypti and Aedes albopictus (Diptera: Culicidae) with egg surface morphology and morphometrics using scanning electron microscopy. Arthropod Structure and Development 40(5): 479-483.
  27. . Bar A, Andrew J (2013). Morphology and morphometry of Aedes aegypti larvae. Annual review and Research in Biology 3(1): 1-21.
  28. . De Araújo I F, De Araújo P H F, Ferreira R M A, Sena I D S, Lima A L, Carvalho J C T, Souto R N P (2018). Larvicidal effect of hydroethanolic extract from the leaves of Acmella oleracea LRK Jansen in Aedes aegypti and Culex quinquefasciatus. South African Journal of Botany 117: 134-140.
  29. . Sharma A, Mishra M, Dagar V S, Kumar S (2022). Morphological and physiological changes induced by Achyranthes aspera-mediated silver nanocomposites in Aedes aegypti larvae. Frontiers in Physiology 13: 1031285.
  30. . Fouad H, Hongjie L, Hosni D, Wei J, Abbas G, Ga’al H, Jianchu M (2018). Controlling Aedes albopictus and Culex pipiens pallens using silver nanoparticles synthesized from aqueous extract of Cassia fistula fruit pulp and its mode of action. Artificial cells, nanomedicine, and biotechnology 46(3): 558-567.
  31. . Pant R, Gupta D K (1979). The effect of exposure to low temperature on the metabolism of carbohydrates, lipids and protein in the larvae of Philosamia ricini. Journal of Biosciences 1: 441-446.
  32. . Lomate P R, Sangole K P, Sunkar R, Hivrale V K (2015). Superoxide dismutase activities in the midgut of Helicoverpa armigera larvae: identification and biochemical properties of a manganese superoxide dismutase. Open Access Insect Physiology :13-20.
  33. . Fal S, Aasfar A, Rabie R, Smouni A, Arroussi H E (2022). Salt induced oxidative stress alters physiological, biochemical and metabolomic responses of green microalga Chlamydomonas reinhardtii. Heliyon 8(1).
  34. . Farghl A M, Shaddad M A K, Galal H R, Hassan E A (2015). Effect of salt stress on growth, antioxidant enzymes, lipid peroxidation and some metabolic activities in some fresh water and marine algae. Journal of Botany 55(1): 1-15.
  35. . Oliver S V, Brooke B D (2016). The role of oxidative stress in the longevity and insecticide resistance phenotype of the major malaria vectors Anopheles arabiensis and Anopheles funestus. PloS one 11(3): e0151049.
  36. . Dias F A, Gandara A C, Perdomo H D, Gonçalves R S, Oliveira C R, Oliveira R L, Oliveira P L (2016). Identification of a selenium-dependent glutathione peroxidase in the blood-sucking insect Rhodnius prolixus. Insect biochemistry and molecular biology 69: 105-114.
  37. . Maheshwari D T, Kumar M Y, Verma S K, Singh V K, Singh S N (2011). Antioxidant and hepatoprotective activities of phenolic rich fraction of Sea buckthorn (Hippophae rhamnoides L.) leaves. Food and chemical toxicology 49(9):2422-2428.
  38. . Haleem D R A, El Tablawy N H, Alkeridis L A, Sayed S, Saad A M, El- Saadony M T, Farag S M (2022). Screening and evaluation of different algal extracts and prospects for controlling the disease vector mosquito Culex pipiens L. Saudi Journal of Biological Sciences 29(2): 933-940.
  39. . Sheehan D, Meade G, Foley V M, Dowd C A (2001). Structure, function and evolution of glutathione transferases: implications for classification of non- mammalian members of an ancient enzyme superfamily. Biochemical journal 360(1): 1-16.
  40. . Adesina J M (2023). Antioxidant and detoxifying enzymes response of stored product insect pests to bioactive fractions of botanical extracts used as stored grains protectant. Annals of Environmental Science and Toxicology 7(1): 043-051.
  41. . Muthusamy R, Ramkumar G, Karthi S, Shivakumar M S (2014). Biochemical mechanisms of insecticide resistance in field population of Dengue vector Aedes aegypti (Diptera: Culicidae). International Journal of Mosquito Research 1(2):1-4.
  42. . Oliver S V, Brooke B D (2014). The effect of multiple blood-feeding on the longevity and insecticide resistant phenotype in the major malaria vector Anopheles arabiensis (Diptera: Culicidae). Parasites and vectors 7: 1-12.
  43. . Engdahl C, Knutsson S, Fredriksson S Å, Linusson A, Bucht G, Ekström F (2015). Acetylcholinesterases from the disease vectors Aedes aegypti and Anopheles gambiae: Functional characterization and comparisons with vertebrate orthologues. PLoS One 10(10): e0138598.