Vol. 24 No. 4 (2025): Mapana Journal of Sciences
Research Articles

Evaluation of the Chemoinhibitory effects of Some Fruits found in Manipur on Kidney stone in in vitro and comparison of the effects with the herbal drug - Cystone.

  • Ibopishak Singh Oinam,
  • Dr. Asem Bimola Devi,
  • Leichil Oinam,
  • Dr. Khundrakpam Nehru Singh,
  • Sonia Thiyam
Ibopishak Singh Oinam
Manipur University
Dr. Asem Bimola Devi
Manipur University
Bio
Leichil Oinam
Delhi University
Bio
Dr. Khundrakpam Nehru Singh
Manipur Iniversity
Bio
Sonia Thiyam
Dr. Pranjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharastra
Bio

Published 2025-12-01

Keywords

  • medicinal plants,
  • kidney stone disease,
  • antiurolithiatic property,
  • calcium oxalate stone,
  • statistical correlation,
  • inhibitory effect
    • ...More
    Less

Copyright (c) 2025

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Abstract

Now-a-days, most people are concerned about their health. Good health means almost free from all kind of diseases. Among these diseases, kidney stone disease is one of the most common ailments (3rd place) in the world. The most emerging technique for the treatment of kidney stone is the use of medicinal plants(leaf, fruit, flower, whole plant, etc) because it is more economic and fewer side effect. The present research work is the study of the effects of locally available fruits of Manipur on kidney stones formation(mainly calcium oxalate stone formation) both in the aqueous and urinary media. Fruits are providing antioxidants, antibiotics, antiurolithiatic property, etc. The statistical correlation study report has that when investigating the effect of fruit extract in aqueous and urinary media, a highly significant linear relationship was observed between Inhibition (0.1%) and the concentration of Ca2+ in solution (g). However, weak linear relations (P>0.05) were observed between Inhibition (0.1%) and Ca2+ in precipitate (g), as well as between the concentration of Ca2+ in solution (g) and Ca2+ in precipitate (g).Among the fruits studied, Celtis australis has the highest inhibitory effect on calcium oxalate(COX) stone formation in aqueous medium while Citrus latipes has the highest inhibitory effect on COX stone formation in the urinary medium.

References

  1. Dawson CH, Tomson CR, Kidney Stone disease, pathology, investigation and medical treatment. Clin Med(Lond). 2012;12(5):467-71, doi: 10.7861/Clinmedicine.12-5-167.
  2. Moe OW, Kidney stones: pathphysiology and medicinal management. Lancet. 2006;367(9507):333-344. doi: 10.1016/S0140-6736(06)68071-9
  3. Younis A, Radhakrishnan S. Ytends Urol Men’s Health. 2011; 2:15-20. doi: 10.1002/tre.197.
  4. Han H, Segal Am, Seifler JL, Dwyer JT, Nutritional management of kidney stones(Nephrolithiasis) ,Clin Nutr Ress. 2015;4(3):137-152 doi:10.7762/enr.2015.4.3.137.
  5. Mahmoud NAMRKNKSA, Bahmani. Medicinal plants for treatment of kidney stones, An ethnobotany study in Shahrekord. Egyptian J Vet Sci. 2019;50(2): 145-149. doi:10.21608
  6. Alelign T, Petros B, Kidney stone disease: Anupdate on current concepts. Adv Urol. 2018,P.3068365-3068365. doi: 10.1155/2018/3068365.
  7. Coe Fl, Evan A, Worcester E. Kidney stone disease. J Clin Invest. 2005;115(10): 2598-2608. doi: 10.1172/Jcl26662.
  8. Bihl G, Meyers A. Recurrent renal stone disease advances in pathogensis and clinical management. Lancet. 2001;358(9282):651-656. doi: 10.1016/S0140-6736(01)05782-8.
  9. Bhutani KK, Goyal A & Singh S, Herbal Wealth of Northeast India: A pictorial and herbaria guide. Department of Natural Products, National Institute of Pharmaceutical Educational and Research 2008, S.A.S. Nagar, Punjab, Ludhiana. Doi:10.1038.
  10. Yadav RD, Jain SK, Alok S, Mahor A, Barb JP, Jaiswal M. Herbal plants used in the treatment of urolithiasis: a reviw. Int J Pharm Sci. 2011;2(6):1412-1420. DoI: 10.47583
  11. Kasole DM, Jagtap SD, Thapa D, Khyade MS, Russell WR. Herbal remedies for urinary stones used in India and China: A review J Ethanopharmacol. 2017;203:55-68. doi:10.1016/j/jep.2017.03.038.
  12. Coe Fl, Evan A, Worcester E. Kidney stone disease. J Clin Invest. 2005;115(10): 2598-2608. doi: 10.1172/Jcl26662.
  13. Bihl G, Meyers A. Recurrent renal stone disease advances in pathogensis and clinical management. Lancet. 2001;358(9282):651-656. doi: 10.1016/S0140-6736(01)05782-8.
  14. Kasole DM, Jagtap SD, Thapa D, Khyade MS, Russell WR. Herbal remedies for urinary stones used in India and China: A review J Ethanopharmacol. 2017;203:55-68. doi:10.1016/j/jep.2017.03.038.
  15. Anamika U, Chaturvedi SS, Tiwari BK, Department of Environmental Studies, NEHU, Shillong –793022, Meghalaya, Utilization of wild Citrus by Khasi and Garo tribes of Meghalaya, Mohammed I, Rasool, Tariq H. Mousa Z, Hayjaa M. Jan2016; 15(1):121- 7, doi: 10.56042.
  16. Muharrem, Lutfi & Ugur C, An ethanobotanical survey of medicinal plants in Karliova(Bingo-Turkey), Ind.J.Tradit.Know. 2019 ;18(1):76-87, doi:10.56042.
  17. Sheikh Z , Raees K , Alushtaq A , Muhammad ZB , Muhammad Z. Ethanobotanical survey of highly effective medicinal plants and phytotherapies to treat diabetes mellitus II in South-west Pakistan, Ind.J.Tradit.Know.2018 ; 17(4): 682-690, doi:10.56042.
  18. Mohammed I, Rasool, Tariq H. Mousa Z, Hayjaa M. Potential of medicinal plants for the management of renal stones, Bagh. J. of Biochem. and Appl. Bio. Sci.2022; 3(2):69-98(e-ISSN,2706-9915),doi:10.47149.
  19. Ankur C, Amarchand P, Aadarsh C, Deepa I, S PR, K PV. Potential of medicinal plants in kidney, gall and urinary stone. Int J Drug Dev Res. 2010;2(2): 431-447. doi: 10.36648.
  20. Basavaraj DR, Biyani CS, Browing Aj, Cartledge JJ. The role of urinary kidney stone inhibitors and promotors in the pathogenesis of calcium containing renal stones. EAU-EBU Update Ser. 2007;5(3)126-136. doi: 10.1016/j-eeus.2007.03.002.
  21. Stamatelou KK, Francis ME, Jones CA, Nyberg LM. Curhan GC. Time trends in reported prevalence of kidney stones in the United States. 1976-1994. Kidney Int. 2003;63(5): 1817-1823. doi: 10.1046/j.1523-1755.2003.00977.
  22. Shu X, Cai H, Xiang YB,. Green tea intake and risk of incident kidney stone: Propective cohort studies in middle aged and elderly chinese individuals. Int J Urol. 2019;26(2):241-246. doi: 10.1111/iju.13849.
  23. Mahmoud N & Bahmani, Medicinal Plants for treatment kidney stones, An ethnobotany study in Shahrekord, Egy.J. vet. Sci.2009;50(2):145-152,doi:10.21608.
  24. Brikowski Th, Lotan Y, Pearls MS, Climate- related increase in the prevalence of Urolithiasis in the United States. Proc.Natl.cad. Sci. USA.2008;105(28):9841-6, doi:10.1073.
  25. Romero V, Akpiner H, Assimos DG, Kidney stones: prevalence, incidence and associated risk factors, Rev.Urol. 2010; 12(2):e86-e96,doi:10.3909.
  26. Bidyalakshmi MD & Ashalata D, Estimation of phytochemical constituents and antioxidant potential of underutilized wild edible fruits of Manipur, Mohammed I, Rasool, Tariq H. Mousa Z, Hayjaa M.May2024 ;33(5):478-488, doi: 10.56042/ijtkv23i5.11041.
  27. Aggarwal KP, Narula S, Kakkar M &Tandon C. Nephrosis: molecular mechanism of renal stone formation and the critical role played by modulators. Biomed.Res.Int.2013;292953-55. Available from, doi:10.1155/2023/292953
  28. Carvalho M ,Nakagawa Y. Urinary supersaturation and recurrence in nephrolithiasis. Int. Braz. Urol.1999; 25:475-9, doi:10.1590.
  29. Curhan GC, Willett WC, Knight EL, Stampfor MJ. Dietary factors and the risk of incident kidney stones in younger women: Nurses’ Health Study II, Arch. Inter. Med. 2004:164:885-91, doi:10.1001/archinte.164.8.885[PubMed]{CrossRef][Google Scholar.
  30. Taylor EN, Stampfor MJ, Curhan GC, Dietary factors and the risk of incident kidney stones in men: New insight after 14 years of follow up. J.Am.Soc.Nephrol.2004:15:3225-32, doi: 10.1681
  31. Meschi T., Nouvenne A, Ticinesi A, Prati B, Guerra A .Dietary habits in women with recurrent idiopathic calcium nephrolithiasis, J.Transl.Med.2012;10:63. doi:10.1186/1479-5876-10-63[PMC free article][PubMed][CrossRef][Google Scholar].
  32. Mina CN, Marziyeh IH, Roja R, Mohammad HSZ, Seyed MN & Anupam B.Dietary plants for the prevention and management of kidney stones: Preclinical evidence and molecular mechanisms, Int. J. Mo. Sci. Mar2018 19(3):765.doi:10.3390.
  33. Meschi T, Maggiore U. Fiaccadori E., Schianchi T., Bosi S.The effect of fruits and vegetables on urinary stone risk factors. Kidney Int. 2004; 66:2402-10. doi:10.1111/j.1523-1755.2-04.66029.x.[PubMed][CrossRef][Google Scholar].
  34. Bhagavathula AS, Al-Khatib M, Elnour Aj, AA, Kalbani A, Shehab NM, et al. Ammi visnaga in treatment of urolithiasis and hypertriglyceridemia. Pharmconosy Res.2014;7(4):397-400. doi:10.4103/0974-8490-167894.
  35. Kumar V, Sinha AK, Makhar HP & Becker K.Dietary roles of phytate and phytase in human nutrition: A review. Food Chem.2010;120:94559.doi:10.1016/j.foodchem.2009.11.052[CrossRef][Google Scholar]
  36. Seltzer MA, Low RK, McDonald M, Shami GS & Stoller ML. Dietary manipulation with lemonade to treat hypocitraturic calcium nephrolithiasis, J. Urol. 1996;156:907-9.doi:10.1097.
  37. Sorensen MD, His RS, Health initiative report. J. Urol. 2014;192:1694-99. doi:10.1016/j.Juro.2014.05.086[PMC free article][PubMed][CrossRef][Google Scolar]
  38. Rao TVRK , Choudhury VK. Chemoinhibition of Minerealization of Urinart stone formation by some inorganic and organic salts of Aluminium in Aqueous and Urinary Medi, Asian J. of Chem. 2008 ;20(7):5046 -52. doi: 1014233.
  39. Basavaraj DR, Biyani CS, Browing Aj, Cartledge JJ. The role of urinary kidney stone inhibitorsand promotors in the pathogenesis of calcium containing renal stones. EAU-EBU Update Ser. 2007;5(3)126-136. doi: 10.1016/j-eeus.2007.03.002.
  40. Ramesh KS, Arina R, Kavita S, Parchuri P, Xiaomin S. Bioactive compounds of citrus fruits: A review of composition and health benefits of carotenoids, flavonoids, limonoids and terpenes. Antioxidant(Basel), Feb2022;11(2):239, doi:10.3390/antiox11020239.
  41. Ranjan S, Gokila M, Brinda P & Sujatha RK. Antioxidant and Phytochemical properties of Aegle marmelos fruit pulp, Int. J. of Current Pharm. Res. 2011;3:65-70, doi:10.22159.
  42. Goa Y, Huany R, Gong Y, Park HS, Wen Q. The Antidiabetic Compounds, 2-dodecyl-6-methoxycyclohexa-2,5-diene- 1,4-dione, Isolated from Averrhoa carmbola L., Demonstrates significant Antitumor potential against Human Breast Cancer Cells. Oncotarget. 2015; 6(27):24304-24319, doi:10.18632/ontarget.4475.
  43. Xie, Q, Zhang, S, Chen, C, Li, J, Wei, X, Xu,X. Protective Effect of 2-dodecyl-6-methoxycyclohexa-2,5-diene- 1,4-dione, Isolated from Averrhoa carambola L., Against Palmitic acid-Induced inflammation and Apoptosis in Min6 Cells by inhibiting the TLR4 – MyD88-NF-KB Signalling Pathway, Cell Phsio. Biochem.2016;39(5):1705-15, doi:10.1159/000447871.
  44. Chen CX, Nong ZH, Xii QQ, He JH, Cai WE, Tang. 2-dodecyl-6-methoxycyclohexa-2,5-diene- 1,4-dione inhibits the Growth and Metastasis of Breast Carcinoma in Mice. Sci.Rep. 2017;7(1):6704. doi:10.1038/41598-017-07162-3.
  45. Bimola DA, Warjeet SL, Ibopishak SO, Jeena Th. The isolation of compounds from the aqueous methanol extract of Cissus javana DC leaves and determination of its trace element content through wet digestion. Asian J.of Chem. 2016; 26(13):3820-3822, doi: 10.14233.
  46. Oinam IS, Asem BD, Rajkumar HS. Evaluation of the effects of Orthosiphon spiralis(Lour) Merr., Cissus Javana DC., and Upupa epops L., on kidney stone along with cystone – a herbal drug. J. Trad. Folk Pract. 2023;10(01&02):10-7, doi:.10.25173.