An Efficient Method of Production of Colloidal Chitin for Enumeration of Chitinase Producing Bacteria

  • Shaun Joe Christ University
  • Suma Sarojini Christ University


Chitin is a long-chain polymer of an N-acetyl glucosamine, a derivative of glucose, and is one of the most abundant natural polymer which has proved versatile for several medical, industrial and biotechnological purposes. Chitinase enzymes have received wide spread attention for its biotechnological applications mainly in the field of agriculture as a bio control agent against fungi and certain insects. An efficient way of obtaining colloidal chitin and the enumeration of chitinase producing bacteria with fungicidal properties were the main objectives of the current study. For this purpose two different species of fungi, Alternaria sp. and Fusarium sp. were isolated from infected vegetables. Chitinase can be used as a potential alternative to chemical fungicides. The current study elucidates an effective method of preparation of colloidal chitin to enumerate the chitanase producing bacteria.


[1] P. H. Clarke and M. V. Tracy, “The Occurrence of Chitinase in some Bacteria”, Journal of General Microbiology, Vol. 14, pp.188-196, 1956.
[2] M. S. Brzezinska, U. Jankiewicz, A. Burkowska and M. Walczak, "Chitinolytic Microorganisms and Their Possible Application in Environmental Protection", Current Microbiology , Vol. 68, pp.71–81, 2014.
[3] N. Murthy and B. Bleakley, "Simplified Method of Preparing Colloidal Chitin Used For Screening of Chitinase- Producing Microorganisms", The Internet Journal of Microbiology, vol. 10, issue2, pp.7, 2012.
[4] S. Krithika and C. Chellaram, “Isolation, Screening, and Characterization of Chitinase producing bacteria from Marine wastes”. International Journal Pharmaceutical Sciences, Vol 8, Issue 5, pp.34-36, 2016.
[5] Woo, Cheol-Joo, Un-Jung Yun and Heui-Doing Park, “Isolation of chitin-utilizing bacterium and production of its extracellular chitinase”, Journal of Microbiology and Biotechnology, Vol. 6, No. 6, pp.439-444, 1996.
[6] Saima, M. Kuddus, Roohi and I. Z. Ahmad, "Isolation of novel chitinolytic bacteria and production optimization of extracellular chitinase", Journal of Genetic Engineering and Biotechnology, Vol. 11, pp.39–46, 2013.
[7] Kirk P M, Cannon P F, Minter DW, Stalpers J A , Dictionary of the Fungi, 10th edition, Wallingford: CABI. p. 22, 2008.
[8] Nelson, P E; Dignani, M C; Anaissie, E J. "Taxonomy, biology, and clinical aspects of Fusarium species". Clin Microbiol Rev. Vol.7, no. 4, pp.479–504, 1994.
[9] Thurston, D., Tropical plant diseases. Second Edition, APS Press. The American Phytopathological Society. St. Paul, Minnesota, USA, 1998.
[10] J. Wang, J. Zhang, F. Song, T. Gui and J. Xiang, "Purification and Characterization of Chitinases from Ridgetail White Prawn Exopalaemon carinicauda", Molecules, vol. 20, no. 2, pp. 1955-1967, 2015.
How to Cite
JOE, Shaun; SAROJINI, Suma. An Efficient Method of Production of Colloidal Chitin for Enumeration of Chitinase Producing Bacteria. Mapana - Journal of Sciences, [S.l.], v. 16, n. 4, p. 37-45, oct. 2017. ISSN 0975-3303. Available at: <>. Date accessed: 20 jan. 2019. doi:
Research Articles