Molecular Identification of Dysphania Militaris, Tirumala Septentrionis and Euploea Core Based on Mitochondrial COI Gene
Keywords:
Molecular identification, Dysphania militaris, Tirumala septentrionis, Euploea core, Mitochondrial CO I geneAbstract
Butterflies and moths belong to the second largest insect order, Lepidoptera, enclosing 46 super families and 126 families. Out of 15000 species of butterflies recorded globally, India harbours 1500 species, of which 316 species are found in Kerala. The morphological studies of Dysphania militaris (Family: Geometridae), Tirumala septentrionis (Family: Nymphalidae), Euploea core (Family: Nymphalidae) enabled the identification of the specimens up to species level and molecular confirmation was done by the analysis of mitochondrial CO1 gene. Genomic DNA, isolated from leg tissue extracts using a Genomic DNA extraction kit, was amplified using PCR specifically for the Mitochondrial encoded cytochrome c oxidase I gene sequences (MT- CO I). The amplified product underwent Agarose gel electrophoresis, visualized under a UV-trans-illuminator, and later, gel elution was conducted. The sequences were analysed using COI gene-specific forward and reverse primers of insects. The BLAST results confirmed similarity with Dysphania militaris, Tirumala septentrionis and Euploea core.
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