Vol. 23 No. 1 (2024)
Research Articles

Molecular Identification of Dysphania militaris, Tirumala septentrionis and Euploea core Based on Mitochondrial COI Gene

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  • Jisha Jacob Valiyaparambil,
  • Aswathi M K,
  • Nandini N J,
  • Manoj Mathews
Jisha Jacob Valiyaparambil
University of Calicut
Aswathi M K
University of Calicut
Bio
Nandini N J
University of Kerala
Bio
Manoj Mathews
University of Calicut
Bio
DOI: https://doi.org/10.12723/mjs.68.8

Published 2024-04-25

Keywords

  • Molecular identification,
  • Dysphania militaris,
  • Tirumala septentrionis,
  • Euploea core,
  • Mitochondrial CO I gene

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Abstract

Butterflies and moths belong to the second largest insect order, Lepidoptera, enclosing 46 super families and 126 families. Out of 15000 species of butterflies recorded globally, India harbours 1500 species, of which 316 species are found in Kerala. The morphological studies of Dysphania militaris (Family: Geometridae), Tirumala septentrionis (Family: Nymphalidae), Euploea core (Family: Nymphalidae) enabled the identification of the specimens up to species level and molecular confirmation was done by the analysis of mitochondrial CO1 gene. Genomic DNA, isolated from leg tissue extracts using a Genomic DNA extraction kit, was amplified using PCR specifically for the Mitochondrial encoded cytochrome c oxidase I gene sequences (MT- CO I). The amplified product underwent Agarose gel electrophoresis, visualized under a UV-trans-illuminator, and later, gel elution was conducted. The sequences were analysed using COI gene-specific forward and reverse primers of insects. The BLAST results confirmed similarity with Dysphania militaris, Tirumala septentrionis and Euploea core.

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