Vol. 24 No. 1 (2025): Mapana Journal of Sciences
Research Articles

Fluorescence Quenching analysis of 6MNPM molecule by Steady State Method

  • Srinath More,
  • Dayanand Lalsangi,
  • Vijayalalaxmi Mallayya,
  • S M Hanagodimath
Srinath More
Gulbarga University Kalaburagi
Dayanand Lalsangi
Gulbarga University, Kalaburagi.
Vijayalalaxmi Mallayya
Gulbarga University, Kalaburagi.
S M Hanagodimath
Gulbarga University, Kalaburagi.

Published 2025-04-05

Keywords

  • Quenching,
  • Steady-state,
  • Stern-Volmer equation,
  • Fluorescence lifespan,
  • Activation energy

Copyright (c) 2025

Creative Commons License

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Abstract

The current work uses aniline as a quencher in a variety of solvents to investigate the room-temperature fluorescence quenching of a physiologically active fluorescent probe, namely “6-Methoxy-4-(4-nitro-phenoxy methyl)-chromene-2-one (6MNPM) coumarin molecule”. To understand its behaviour in various media, several solvents with different dielectric constants and refractive indices have been used. Spectroscopy techniques and time-correlated single photon counting were used to describe the absorption spectra, emission spectra, and lifespan value of a molecule. From fluorescence quenching analysis, we find that the S-V curve shows a linear dependence in given solvents with various dielectric values. It is shown that quenching responses are dynamic, many forms of quenching have been identified, and the relevant parameters have been assessed.

References

  1. Margaret H, Sherlock J, James, Kaminski, 1988 J. Med. Chem. 31 1221-2108.
  2. Liu C Y, Lin C M, Chem Y J 2012 Toxicol. Appl. Pharm. 259 (2) 219-226.
  3. Christos A, Mitsos, Alexandros L Zografos 2003 J. Org. Chem. 68 (11) 4567-4569.
  4. Hoult JR, Paya M (1996) Pharmacological and biochemical actions of simple coumarins: natural products with therapeutic potential. General Pharmacology: The Vascular System 27:713-722.
  5. Matos M J, Vina D, Vazquez-Rodriquez S, Uriarte E, Santana L (2012). Focusing on new monoamine oxidase inhibitors: differently substituted coumarins as an interesting scaffold. Curr Top Medical Chem 12:2210-2239.
  6. Matos J, Vazquez-Rodriquez S, Santana L, Uriarte E, Fuentes-Edfuf C, Santos Y, Munoz-Crego A (2013) Synthesis and structure-activity relationship of novel antibacterial agents. Molecules 18:1394-1404.
  7. Morsy S A, Farahat A A, Naser M N A, Tantawy AS (2017) Synthesis, molecular modeling and anticancer activity of new coumarins containing compounds. Saudi Pharmaceutical Journal 25:873-883.
  8. Xia L, Wang Y, Hung W, Qian H (2013) Research advance of anticancer drugs with coumarin structures. Chenese Journal of New Drugs 22:2392-2404.
  9. Munir S, Shah A A, Shahid M, Ahmad M S, Shahid A, Rajoka MSR, Akash MSH, Akram M, Khurshid M (2020) Antiangiogenesis potential of phytochemicals for the therapeutic Management of Tumours. Curr Pharm Des 25:265-278.
  10. Song Y, Chen Z, Li H (2012) Advances in coumarin-derived fluorescent chemosensors for metal ions. Curr Org Chem 16:2690-2707.
  11. Fujian Huang, Fan Xia (2018) Fluorescence sandwich assays for protein detection, biosensors based on sandwich assays, 29-45.
  12. Lackowicz J R, (2006) Principle of fluorescence spectroscopy, 3rd edition. Plenum Press, New York.
  13. Lackowicz J R, (2006) Principle of fluorescence spectroscopy, 3rd edition. Plenum Press, New York.
  14. Varsha V Koppal, Raveendra M Melavanki, Raviraj A Kusanur, and Ninganagouda R Patil (2018) Bimolecular fluorescence quenching reactions of the biologically active coumarin composite 2-acetyl-3H-benzo[f] chromen-3-one in different solvents, Luminescence, 1-7.
  15. Nad S, Pal H (2000) Electron transfer from aromatic amines to excited coumarin dyes: fluorescence quenching and picosecond transient absorption studies. J Phys Chem A 104:673-680.
  16. Mahantesha Basanagouda, Manohar V Kulkarni, Deepak Sharma, Vivek K Gupta, Pranesha, P Sandhyarani, Vijaykumar P Rasal. J Chem Sci (2009) 121:485-495.
  17. Nagaraja D, Melavanki RM, Patil NR (2013) Fluorescence quenching of 6BDTC by aniline in different solvents. Cana J Physics 91:966-970.
  18. Koppal VV, Patil PG, Melavanki RM, Kusanur RA, Patil NR (2019) Solvent effect on the relative quantum yield and preferential solvation of biologically active coumarin derivative. Macro mol Symp 387:1800210-1800212.
  19. Patil NR, Melavanki Rm, Kapatkar SB, Chandrashekar K, Umapathy S (2011) Fluorescence quenching of biologically active carboxamide by aniline and carbon tetrachloride in different solvents using Stern-Volmer plots. Spectrochim Acta A Mol Bio Mol Soectrosc 79: 1985-1991.
  20. Andre JC, Niclause M, Ware WR (1978) Kinetics of partly diffusion-controlled reactions. I. Transient and apparent transient effect in fluorescence quenching. Chemical Physics 28:371-377.
  21. VV Koppal, Raveendra Melavanki, Raviraj Kusanur, NR Patil (2022) Analysis of fluorescence quenching of coumarin derivative under steady state and transient state methods. Journal of Fluo (2021) 31:393-400.
  22. Wilemski G, Fixman M (1973) General theory of diffusion-controlled processes reactions. Journal of Chem Phy 58:4009-4019.
  23. Yugerabide J, Dillon MA, Burton M (1964) Kinetics of diffusion-controlled processes in liquids. Theoretical consideration of luminescent systems: quenching and excitation transfer in collision. Journal of Chemical Physics 40:3040-3052.
  24. Edward J T (1970) Molecular volumes and the stokes-Einstein equation. Journal of Chemical Education 47:261-270.
  25. Sharma VK, Mohan D, Sahare PD (2007) Fluorescence quenching of 3-methyl-7-hydroxyl coumarin in the presence of acetone. Spectrochim Acta A 66:111-113.
  26. Williamson B, La Mer VK (1948) The Kinetics of activation-diffusion controlled reactions in solution; the temperature dependence of the quenching of fluorescence. Journal of American Chemical Society 70:717-721.
  27. Omnath Patil, P.K. Ingalagondi, Shivaraj Gounhalli, and S.M. Hanagodimath. 2020. Dipole moments and quenching of fluorescence of a new coumarin dye. Canadian Journal of Physics. 98(8): 761-769. https://doi.org/10.1139/cjp-2019-0517.
  28. Srinath, Patil, O., Devar, S. et al. Estimation of Electric Dipole Moment by Solvatochromism, Computational Method, and Study of the Effect of Solvents by Preferential Solvation of 6 – Methoxy—4—(4—Nitro—Phenoxy Methyl)—Chromen—2—One (6mnpm). J Fluoresc (2024). https://doi.org/10.1007/s10895-024-03955-8