Vol. 24 No. 1 (2025): Mapana Journal of Sciences
Research Articles

Fluorescence Quenching Analysis of 6MNPM Molecule by Steady State Method

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  • Srinath,
  • Dayanand Lalsangi,
  • Vijayalalaxmi Mallayya,
  • S M Hanagodimath
Srinath
Department of PG Studies and Research in Physics Gulbarga University, Kalaburagi – 585 106, Karnataka, India
Dayanand Lalsangi
Department of PG Studies and Research in Physics Gulbarga University, Kalaburagi – 585 106, Karnataka, India
Vijayalalaxmi Mallayya
Gulbarga University, Kalaburagi.
S M Hanagodimath
Department of PG Studies and Research in Physics Gulbarga University, Kalaburagi – 585 106, Karnataka, India
DOI: https://doi.org/10.12723/mjs.72.3

Published 2025-04-05

Keywords

  • Quenching,
  • Steady-state,
  • Stern-Volmer equation,
  • Fluorescence lifespan,
  • Activation energy

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Abstract

The current work uses aniline as a quencher in a variety of solvents to investigate the room-temperature fluorescence quenching of a physiologically active fluorescent probe, namely “6-Methoxy-4-(4-nitro-phenoxy methyl)-chromene-2-one (6MNPM) coumarin molecule”. To understand its behaviour in various media, several solvents with different dielectric constants and refractive indices have been used. Spectroscopy techniques and time-correlated single photon counting were used to describe the absorption spectra, emission spectra, and lifespan value of a molecule. From fluorescence quenching analysis, we find that the S-V curve shows a linear dependence in given solvents with various dielectric values. It is shown that quenching responses are dynamic, many forms of quenching have been identified, and the relevant parameters have been assessed.

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